Cyclin e he12
WebMCM7 (141.2), anti-cyclin E (HE12), anti-cyclin A (H-432), anti-cyclin B (H-433), and anti-CDK2 (M2) antibodies were purchased from Santa Cruz Biotechnology. Anti-ORC2 and anti- -actin antibodies were pur-chased from BD Biosciences and Sigma, respectively. Anti-p53 antibody was obtained from Cell signaling technology. Anti-pRB antibody was WebProduct Details Product Specific Information Description: The monoclonal antibody HE12 recognizes human Cyclin E. Cyclins regulate the cell cycle by binding and activating …
Cyclin e he12
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WebJun 24, 2002 · Human cyclin A was detected with antibody CY-A1 (Sigma), cyclin E with antibody HE12 (Santa Cruz Biotechnology), XCdc6 with polyclonal antibody hCdc6 (ref. 17), Mcm2 with monoclonal antibody BM28 ... Webcyclin E 항체 (HE12) 는 마우스 monoclonal IgG 1 κ, 775간행물에 인용, 이며 200 µg/ml 으로 제공합니다. human origin의 cyclin E의 전체인 아미노산을 항원으로 사용하였습니다. 안티 …
WebCyclin E binds and activates the kinase Cdk2 and by phosphorylating its substrates, the so-called "pocket proteins", the cyclic/Cdk2 complexes initiate a cascade of events that leads to the expression of S-phase specific genes. Aside from this specific function as a regulator of S-phase-entry, cyclin E plays a direct role in the initiation of ... WebNO donor was associated with the down-regulation of cyclin D1 and hypophosphorylation of the retinoblastoma protein. No changes in the levels of cyclin E or the catalytic partners of these cyclins, CDK2, CDK4, or CDK6, were observed. This NO-induced cytostasis and decrease in cyclin D1 was reversible for up to 48 h of DETA-NONOate (1 mM) treatment.
WebOct 7, 2024 · Previous experiments demonstrated that cyclin D-CDK4/6 and cyclin E/A-CDK2 induce Rb phosphorylation 9,13,17,18,19,20. However, the timing of Rb/E2F … WebDownload Zoom Anti-Cyclin E Antibody, clone HE12 is a high quality Mouse Monoclonal Antibody for the detection of Cyclin E & has been validated in IP, WB, ICC. Anti-Cyclin …
WebSep 11, 2024 · We also observed that the Cyclin E antibody utilized here, the widely used clone HE12, detects a strong nonspecific signal in MCF10A cells, in addition to detecting Cyclin E (S6A and S6B Fig). Thus the difference in Cyclin E signal between CDK2 low and CDK2 inc cells may be partly obscured by the nonspecific signal.
WebSep 24, 2024 · Instead, increased expression of the full-length form of cyclin E1 (nucleus localized) was detected by western blotting using the monoclonal anti-cyclin E antibody (clone HE12), which was... 1等级玩家小说WebThe phosphodegron of cyclin E, LpTPPXpSG, is remarkably similar to that of Myc (12). In the case of cyclin E, the phosphorylation events are catalyzed by Cdk2 (Ser384) and GSK3 (Thr380) (11), respectively, and x-ray structural studies have shown that phospho-Thr380 plays a crucial role in the binding of cyclin E by Cdc4 (3, 12). As is the case for 1等級でも入れる自動車保険WebCyclin E1 (HE12) Mouse mAb: Western Blotting; H Mk: ... Cyclin E/CDK2 further phosphorylates Rb to allow progression into S-phase, where cyclin E/CDK2 is thought to phosphorylate and activate multiple proteins involved in DNA synthesis (2,3). Turnover of cyclin E is largely controlled by phosphorylation that results in SCFFbw7-mediated ... 1等级玩家漫画免费阅读WebMay 15, 1999 · Monoclonal anti-p53 Pab1801, polyclonal anti-cyclin D1 DCS6, monoclonal anti-cyclin E HE12, and monoclonal anti-Rb OP-66 antibodies were used. Cyclin D1 was not expressed in normal bronchial epithelium but was detected in 7% of SMs, 15% of atypias; 18% of LGDs, 47% of HGDs, and 42% of SCCs. 1等级玩家漫画免费看Webcyclin E Antibody (HE12) is an IgG1 κ mouse monoclonal cyclin E antibody suitable for the detection of cyclin E (doublet at 50 kDa and single band at 42 kDa) of mouse, rat and … 1答Webcyclin A (H432) and cyclin E (HE12 and HE111 for immunoblot-ting and immunoprecipitation, respectively) were from Santa Cruz Biotechnology (Santa Cruz, CA). Anti-phospho-CDK2 (Thr160) and ... The HA-cyclin E expression plasmid was constructed by sub-cloning the full-length cyclin E into pcDNA3.1 vector, and is expressed as an N … 1等级玩家漫画在线观看WebNov 25, 2024 · cyclin E Antibody (HE12) is an IgG1 κ mouse monoclonal cyclin E antibody suitable for the detection of cyclin E (doublet at 50 … 1等级玩家韩漫