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Peasy-t5 zero cloning kit

WebTransGen biotech co peasy t5 zero cloning kit Peasy T5 Zero Cloning Kit, supplied by TransGen biotech co, used in various techniques. Bioz Stars score: 88/100, based on 1 … Web关于其他常用耗材的网上超市合同公告采购信息，招标信息，机电设备采购平台

pEASY-Blunt Simple (linearized) Sequence and Map - SnapGene

WebFeb 26, 2024 · The amplicons of the secondary PCR were purified and then cloned into pEASY-T5 Zero Cloning Vector. At least three independent positive clones of each fragment were sequenced by Hangzhou Ykang Biological Technology and Service, and sequence alignment and splicing were done using DNAMAN software. WebJan 8, 2016 · The recovered PCR products were cloned using the pEASY-T5 Zero Cloning Kit (TransGen Biotech Co., Ltd, Beijing, China) by following the product manual. Two positive final clones were submitted to Invitrogen Corporation (Guangzhou, China) for sequencing. Prokaryotic Expression and SDS-PAGE Analysis. read html data in python https://tammymenton.com

Cloning Vectors and Kits Civic Bioscience

Webkit (TiaP., Ltd, Beijing, China), according to the man-ufacturer’s instructions. The recovered PCR products were cloned using the pEASY-T5 Zero Cloning Kit (TransGen Biotech (Beijing) Co., Ltd, Beijing, China) by following the product manual. Two positive final clones were submitted to Invitrogen Corporation (Guangzhou, China) for sequencing. WebJul 30, 2024 · Recombination plasmid of HA gene of H5 subtype has been constructed using the pEASY™-T5 Zero Cloning Kit. The plasmid was cleaved using a suitable restriction enzyme. The digested plasmid was purified with a DNA recovery kit, the concentration was measured with a Nano Drop nucleic acid quantifier, and then an appropriate amount was … WebMay 28, 2024 · The products were ligated to T5 zero cloning vector and transformed with Trans1-T1 into Competent cells by the heat-shock process at 42°C for 30 s (pEASY®-T5 Zero Cloning Kit). Three colonies were randomly picked from each transformation and incubated in Luria-Bertani liquid media at 37°C overnight under continuous shaking. One … how to stop recu

CT501-02 pEASY®-T5 Zero Cloning Kit Biotrend

Category:pUC19L Sequence and Map - SnapGene

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Peasy-t5 zero cloning kit

pEASY®-T5 Zero Cloning Kit - CT501 Civic Bioscience

WebpEASY-Blunt Zero (linearized) Cloning vector for blunt-ended PCR products, with a lacZα-ccdB suicide gene that allows for selection of recombinant plasmids. Sequence Author: … WebOct 1, 2016 · pEASY®-T5 Zero Cloning Kit – CT501. $ 338.00 – $ 673.00 CAD. 0 in stock (expect 7-21 days for delivery) Format. You will earn 16$ if you order this product! $ …

Peasy-t5 zero cloning kit

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WebProduct Details. pEASY® -Blunt Simple Cloning Vector eliminates the multi-cloning sites of pEASY® -Blunt Cloning Vector. It is designed for cloning and sequencing Pfu-amplified PCR products. •5 minutes fast ligation of Pfu-amplified PCR products. •Kanamycin and Ampicillin resistance genes for selection. •Easy blue/white selection. WebpEASY ®-T5 Zero Cloning Vector contains a suicide gene. Ligation of PCR fragment disrupts the expression of the gene. Cells that contain non-recombinant vector are killed …

WebMay 20, 2024 · The PCR products obtained were purified and ligated into the vector pEASY -T5 and subsequently recombinant plasmids were used to transform E. coli Trans 1-T1 competent cells using a pEASY -T5 Zero Cloning Kit (TransGen Biotech, Beijing, China). Both of the recombinant plasmids were verified by sequencing. WebLinearized version of pUC19 for use with the GeneArt® Seamless Cloning and Assembly Kit. Linearized version of pUC19 for use with the GeneArt® Seamless Cloning and Assembly Kit. Features; Plasmids; ... pEASY-T5 Zero (linearized) pKF 18k-2. pSMART LCKan (linearized) CMV promoter. pEX-1. pKF 19k-2. pSP64 Poly(A) GAL1 promoter. pEX-A. pKOV ...

WebTransGen Biotech pEASY®-T5 Zero Cloning Vector contains a suicide gene. Ligation of PCR fragment disrupts the expression of the gene. Cells that contain non-recombinant vector … WebThe Zero Blunt™ PCR Cloning Kit uses the multipurpose cloning vector pCR™-Blunt and ExpressLink™ T4 DNA Ligase to generate a ligation product in a five-minute, room-temperature ligation step. The Zero Blunt™ PCR Cloning Kit is designed to clone blunt PCR fragments (or any blunt DNA fragment) with a low background of non-recombinants ...

WebpEASY® -T5 Zero Cloning Kit Catalog Number：CT501-01 Price：$112.00 Specification： 20 rxns 60 rxns Quantity： Manual Vector Information MSDS COA Inquire Now Buy Now …

WebJan 22, 2024 · The product was cloned into the pEASY-T5 Zero Cloning vector (TransGene Biotech, Beijing, China) for sequencing. For 5′ RACE, the gene specific primers were designed based on the 3′ cDNA end sequence. The 5′ RACE cDNA template was synthesized by SMARTer@RACE cDNA Amplification Kit (TaKaRa, Tokyo, Japan). how to stop recording tiny taskWebFree Trial pEASY-Blunt Simple (linearized) Cloning vector for blunt-ended PCR products, with blue-white selection, kanamycin and ampicillin resistance markers, and a T7 promoter but … how to stop recording series on dish tvWebpEASY® -T5 Zero Cloning Kit 目录号： CT501-01 单 价： ￥780 规格： 20 rxns 60 rxns 数量： - + 说明书 载体信息 质检报告 立即下单 加入购物车 产品详情介绍 该载体通过自杀基因 … how to stop recordings on dish dvrWebpEASY® -T5 Zero Cloning Kit pEASY®-T5 Zero Cloning Vector contains a suicide gene. Pricing & AvailabilityDownload CatalogueAdd to wishlist View More Details Add to Cart pEASY® -Uni Seamless Cloning and Assembly Kit No restriction enzyme digestions, > 95% cloning efficiency, and no extra sequences introduced. read how you want publisherWebEvaluation of pEASY- Uni Seamless Cloning and Assembly Kit to clone multiple fragments of Elaeis guineensis DNA. ... The pEASY™-T5 Zero Cloning Vector (TransGen, Beijing, China) was used for DNA ... read http headersWebpEASY ®-T5 Zero Cloning Vector contains a suicide gene. Ligation of PCR fragment disrupts the expression of the gene. Cells that contain non-recombinant vector are killed … read html table in pandasWebrecovered using a DNA recovery kit (Tiangen Biotech Co., Ltd, Beijing, China). The recovered PCR products were cloned using the pEASY-T5 Zero Cloning Kit (TransGen Biotech Co., Ltd, Beijing, China) by following the product manual. Two positive final clones were submitted to Invitrogen Corporation (Guangzhou, China) for sequencing. how to stop recording zoom meeting